In Vitro Analysis of Anticancer Potential of Green Okra (Abelmoschus esculentus L.) Polysaccharide Extracts in HeLa Cervical Cancer Cell Line

Abstract

Polysaccharide extract has potential as an anticancer agent. Abelmoschus esculentus is one of the plants that is rich in polysaccharide content, but its anticancer activity is still need explore. This study aimed to explore the anticancer activity of Abelmoschus esculentus crude extract polysaccharide by measure the effect on levels of pro-inflammatory cytokines IL-2, IL-12, TNF-α, IFN-γ in HeLa cell line. This study used HeLa cells as a research model with four groups, including KN (media and DMSO), K+ (doxorobicin µg/mL), P1 (Abelmoschus esculentus L. extract 200µg/mL) and P2 (Abelmoschus esculentus L. extract 400µg/mL). The Abelmoschus esculentus L. was extracted using boiled distilled water. Cytokine levels were analyzed using the ELISA test. The results of cytokine levels showed decreasing in the levels of cytokines IL-2, IL-12, TNF-α, IFN-γ, (P < 0.005). In conclusion, the polysaccharide extract of Abelmoschus esculentus L. has the potential as an anticancer and therapeutic agent for cancer. Research on green okra polysaccharide crude extract as an immunosuppressant is expected to be a potential bio-pharmaceutical source.