The Surface Structure, Chemical Molecules, and Thickness of Aggregatibacter actinomycetemcomitans biofilms by Different Inducers Agent

Abstract

Aggregatibacter actinomycetemcomitans caused oral infection in recent years. It requires advancements in health technology, such as infection and sickness detection kits. This study purposed to provide data to aid in the detection kits. Aggregatibacter actinomycetemcomitans was prepared to be biofilm cultured sample. Then the samples were induced by glucose, lactose, soy protein, and iron. Scanning Electron Microscopy Energy Dispersive X-Ray, Confocal Laser Scanning Microscopy, and Olympus FluoView ver 4.2a were used to analyze the ultrastructural dimension of biofilms. The surface structure of biofilm induced by iron differed from that of biofilms induced by other inducers. O(78.65±10.32) and Cl(1.28±0.21) were the most abundant chemical compounds induced by glucose; S(14.67±4.56) and Fe(37.46±59.62) were induced by lactose; C(25.41±16.36) and N(13.49±9.37) were induced by soy protein; and P(58.53±17.80) was induced by iron. Iron induced the highest amount of polysaccharides (605.641±71.66). Lactose (857.877±70.86) induced the greatest number of bacterium cells. Iron (11.000±1000)nm caused the thickest layer. The biofilm of Aggregatibacter actinomycetemcomitans induced by glucose, lactose, soy protein, and iron are all different.