Kadukkai maathirai (Siddha herbal formulation) reverses liver pathology associated with metabolic dysfunction in high fat diet-induced fatty liver disease – a preclinical study

Abstract

The study explores the hepatoprotective effect of Kadukkai maathirai (KM) in high fat diet (HFD) induced nonalcoholic fatty liver disease (NAFLD) in rats. Total 54 Sprague Dawley rats were used in the study, 9 groups: Group I – IV kept as normal and test drug control and group V - NAFLD disease model- received HFD for 40 weeks. Group VI – IX received HFD for 40 weeks and then test drugs: Group VI – VIII received KM in three different doses for 45 days. Metformin (standard) was administered to Group IX for 45 days. On day 46, the blood and liver tissue were collected for analysis. KM at 36, 144mg/kg and metformin showed a significant decrease in ALP level, all three doses of KM and metformin showed a significant reduction in direct bilirubin levels. A significant improvement in HDL was observed in all doses of KM and metformin-treated groups. Oral glucose tolerance test (OGTT) findings in KM treated test groups showed significantly reduced plasma glucose levels. The KM treated groups and metformin-treated groups showed a reduction in body weight at 47th week, and significantly reduced relative liver weight when compared with the HFD group. Histopathological evaluation of KM treated groups showed normal architecture of central vein and hepatic cords. Portal triads were also generally normal in their location and pattern. No indication of fatty liver. This study confirms the ability of phytoconstituents present in KM in reversing the metabolic dysfunction and liver pathology seen in NAFLD. Further studies are required to evaluate KM as a therapeutic agent.