Effect of Biotin Treatment on the improvement of Lipid Profile and Foam Cells in Dyslipidemia rats

Abstract

Introduction: This study aimed to assess the effects of increasing biotin concentrations on lipid profiles, CRP, and foam cells in Wistar rats with dyslipidemia risks. Materials and Methods: Thirty male Wistar rats (weighing 150-200grams) were divided into five groups and adapted for seven days. The negative control group received standard feed, while the positive control group received a high-fat diet. The treatment groups 1, 2, and 3 received a high-fat diet and biotin at different doses: 1.232mg/kg, 68.39mg/kg, and 97.72mg/kg, respectively, for six weeks. This study employed the colorimetric enzymatic method to examine the lipid profiles, a qualitative approach to examine the CRP, and painting Oil Red O and HE on histology slides to count the foam cells. Results: The negative control group indicated normal levels of lipid profiles and foam cells. The positive control group showed increased lipid profile levels and foam cells. Meanwhile, the treatment groups receiving an increase in biotin concentration showed a decreasing pattern of the foam cells, and their lipid profile levels (total cholesterol, triglycerides, and LDL) decreased. However, the HDL did not reduce. The results of all groups' CRP were negative. The one-way ANOVA test showed significance for the levels of total cholesterol, triglycerides, and LDL. The Kruskal-Wallis test was significant for the number of foam cells (a confidence level of 95%). Conclusion: The biotin treatment significantly improves Wistar rats' lipid profiles and the number of foam cells. However, the doses did not statistically affect the levels of HDL and CRP.