Antimicrobial activity of Biosynthesized Silver Nanoparticles of Bauhinia racemosa leaf extracts

Abstract

Increased wettability or solubility of nanoparticles (NPs) can greatly improve their antibacterial activities. The effect of the stabilising agent on the solubility of silver NPs (AgNPs) and their antibacterial and antifungal properties were examined in this study. Aqueous and methanolic solutions of Bauhinia racemosa plant leaf extracts were used as bioreductants for making silver NPs. The synthesised AgNPs were first identified by a change in colour from pale green to dark brown and further confirmed by surface plasmonic resonance (SPR) band at 430 nm for aqueous and 360 nm for methanolic extracts using UV-Visible spectroscopy. The size distribution of generated AgNPs in solution was examined using a DLS particle size analyzer, and it was found to be 114 and 122 nm for aqueous and methanolic extract respectively. Bauhinia racemosa AgNPs exhibited significant antibacterial and anti-fungal activities against E. coli, K. pneumonia, P. aeruginosa, S. aureus and Aspergillus niger, demonstrating that they may be utilized in the biomedicine field.