The Study of the Phenolic compounds, Antioxidant activity and Antibacterial effect of Herbal Ethanol extracts of Scorzonera purpurea subsp. rosea

Abstract

The increased demand for medicinal products of natural origin causes the search for new plants with a certain spectrum of pharmacological action. Scorzonera purpurea subsp. rosea is a one of unexplored plants in the phytochemical and pharmacological sense. The aim of this research was to evaluate the phenolic compounds, antioxidant activity and antibacterial effect of ethanolic extracts of Scorzonera purpurea subsp. rosea (EE-Sp). The results of the study of EE-Sp which contained 70% of ethanol (E70) showed the high content of phenolic compounds (2.111mg/mL), flavonoids (0.276mg/mL) and anti-radical activity of 86%, which was determined by the DPPH method, its effective concentration of inhibition IC50 - 0.67mg/mL as well as the strong ferric reducing potential (FRAP method) - 23.58μmol/mL.The HPLC method was used to identify chlorogenic, caffeic, ferulic, rosmarinic acids, luteolin, quercetin, apigenin, apigenin-7-glucoside, and rutin. Chlorogenic acid, luteolin and apigenin were the dominant substances in terms of quantity. Studies of the antibacterial effect of the extract E70 with respect to the test-strains of bacteria Staphylococcus aureus ATCC 25923, Escherichia coli dH5a, Pseudomonas aeruginosa ATCC 9027 showed the effectiveness of the extract compared to control.