Development and Validation of a New HPLC Method for the Estimation of Sulfamoxole in Bulk and Tablet Formulations-Reference-Cited by-同舟云学术

Development and Validation of a New HPLC Method for the Estimation of Sulfamoxole in Bulk and Tablet Formulations

Published:2023-12-29 Issue: Volume: Page:5617-5623
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Karuna Moparthy Kamala¹,Nadh Ratnakaram Venkata²,Prasad Gorumutchu Giri³

Affiliation:

1. Department of Chemistry, Government College for Women (A), Guntur - 522001, India.

2. Industrial Chemical Product Development and Analysis Centre, Department of Chemistry, GITAM School of Science, GITAM Deemed to be University – Bengaluru Campus, Karnataka – 561203, India.

3. Department of Chemistry, A.G & S.G.S College Vuyyuru, Krishna, District - 521165, India.

Abstract

To quantify the sulfamoxole content in bulk as well as tablet formulation, a new isocratic RP-HPLC method was developed and then validated. Kromasil C18 column with a dimension of 250 X 4.6mm was used which was filled with a particle size of 5μ. A mixture containing CH3OH, CH3CN and H2O in the volume ratio of 55, 30 and 15 was used as a mobile phase at room temperature with an optimized flow rate of 1ml/min. UV detector was set at 241nm for sulfamoxole determination. The run time of the current method is ten minutes. Accomplished the forced degradation studies to understand the stability indicating nature of the current method. As per the current ICH guides (Q2R1), validation of the method was conducted.

Publisher

A and V Publications

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