Anticancer Activity of Microwave Assisted Polyphenolic Compounds Extracted from Combinations of Curcuma Longa and Camellia Sinensis against Lung Cancer Cell Line

Abstract

Objectives: Anticancer activity of microwave assisted polyphenolic compounds was extracted from combinations of Curcuma longa and Camellia sinensisby phytochemical analysis. Invitro anticancer activity was determined by using cell line. Methods: By preliminary phytochemical analysis and using uv-visible spectrophotometer total phenolic content was determined. In vitro anticancer activity was found out by MTT assay method. Result: Phenolic compounds are responsible for antioxidant activity. Plant extracts having hydroxyl groups are responsible for facilitating free radical scavenging. Total phenol content in the plant extract Curcuma longa and Camellia sinensisusing the calibration curve, was found to be 1137.45mg of Gallic acid equivalents/g dry weight of extract. The anticancer activity was carried out with lung cancer cell line (A-549) by MTT assay method. When cancer cells were incubated with combination of curcuma longa and camellia sinensis extracts, they induced cytotoxicity in a significant time which implicit the damage to the membrane integrity of cell. Based on the cell disintegration, when the plant extracts were incubated with lung cancer cells and cell death were calculated. The IC50 value of the given test sample of curcuma longa and camellia sinensisand the standard Cisplatin was found to be 22.72µg and 3.89µg, respectively. This is clearly stated that the selection of A-549 cell line might provide a good evidence for the treatment of lung cancer. Conclusion: Based on the results obtained, combinations of Curcuma longa and Camellia sinensis could be used as an anticancer agent.