Derivative Spectroscopic Method and RP-HPLC Method Development and Validation of Levofloxacin hemihydrate-Reference-Cited by-同舟云学术

Derivative Spectroscopic Method and RP-HPLC Method Development and Validation of Levofloxacin hemihydrate

Published:2023-05-31 Issue: Volume: Page:2239-2244
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Sen Sandip¹,Ravindar Bairam²,Jala Sirikonda²,Dharabonia Laxmi²,Rajeshwari Konika²

Affiliation:

1. Department of Pharmaceutical Chemistry, School of Pharmacy, Guru Nanak Institutions Technical Campus, Ibrahimpatnam, Hyderabad – 501506.

2. Department of Pharmaceutical Chemistry, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapakka, Siddipet – 502277.

Abstract

The present work describes the development of a simple, precise and accurate derivative spectroscopic and RP-HPLC method for the estimation of levofloxacin hemihydrate in the tablet dosage form. Levofloxacin hemihydrate showed absorption maxima at 287.36, 287.2 and 296.2nm in zero, first and second-order spectroscopic methods. The RP-HPLC method was optimized by using Orthophosphoric acid: acetonitrile: methanol (50: 20: 30) as solvent at 287nm. The methods showed linearity in the concentration range 1-5 and 2-10 µg/ ml and the correlation coefficient was 0.999 respectively. The method has been validated according to the ICH guidelines. The percentage relative standard deviation was found to be less than 2% in respect of both methods.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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