Tuna Ethanol Extract reduces CRP and Edema in Animal Models of Adjuvant Arthritis

Abstract

Rheumatic arthritis is an inflammatory joint disease caused by an autoimmune process. Tuna contains many nutrients that act as antioxidants and anti-inflammatories in the form of vitamins, minerals, and essential fatty acids. This study aims to determine the effect of Tuna fish extract on inflammation in the legs of animal models of adjuvant arthritis. Twenty-four male Rattus norvegicus which were partitioned into P1 groups of no treatment rats, P2 groups of adjuvant arthritis (AA) rats, were nduced with Complete Freund's Adjuvant (CFA), P3 groups are rats with adjuvant arthritis who were given Thunnus obesus fish ethanol extricate orally for seven consecutive days from the primary day of the emergence of symptoms of AA, and given with the dosage of 12 grams/kg of body weight, beginning on the 22nd day. At the last day of the study, the CRP and swelling feet joints of all groups of rats were measured (on day 29). Tuna ethanol extracts significantly decrease the mean volume of edema (p = 0.034) and CRP levels (p=0.022) in adjuvant arthritis rats. The content of active ingredients in Tuna has been shown to inhibit the inflammatory process through the inhibition of cox-2, resulting in a reduction of the volume of animal leg joint edema and CRP levels in adjuvant arthritis trials. Administration of Tuna fish extract at a dose of 12g/kg BW can reduce leg edema of adjuvant arthritis experimental animals and serum CRP levels of adjuvant arthritis experimental animals.