In Vivo Pharmacokinetic Studies of Acyclovir Gastro Retentive Mucoadhesive Microspheres Aided by Azadirachta indica Fruit Mucilage

Abstract

The study's main goal is to test gastro retentive mucoadhesive Acyclovir (ACR) microspheres for various in vivo characteristics as a follow-up to the work done by Naresh et al (2022). The optimized ACR Azadirachta indica mucoadhesive microspheres (AAMM) were evaluated in vitro using a variety of parameters. Based on previous research findings, the authors used formula-8 (AAMM-8) for in vivo testing. The availability of plasma drugs was tested on six healthy rabbits of both sexes (weighing 2.-2.5kg). By using a parallel design, they were haphazardly parted into two groups of similar size. Later, the study involved HPLC analysis of the drug in the plasma, which required the fabrication of a spiked plasma sample and the development of an analytical procedure (a simple and cost-effective method for ACR).Pharmacokinetic parameters were measured in plasma samples. The study revealed that in vivo animal studies in rabbits showed good levels of ACR. After a single dose of formulation AAMM-8 (8.85mg/kg), the symmetrical mean Cmax values of formulation AAMM-8 (24.36±0.84µg/mL of ACR), were higher than those of ACR. The tmax values of the AAMM-8 were found to be 6 h. The AUC (0-10h) values were observed to be 176.0µg.h/mL, whereas (AUC)0-∞ was observed to be 248.5µg. h/mL. The AUMC readings were observed to be 724.62µg.h/mL. According to the findings, ACR reached systemic circulation faster than pure medication and had acceptable kinetic values.