Comparative Study of 3T3 Fibroblast cells grown in the normal and increased glucose conditions under the influence of Aloe vera and Costus pictus

Abstract

The drugs developed from medicinal plants are to be examined for its positive and negative impact on the human body and the cell culture plays a major role. The elevated glucose levels in diabetic patient leads to complications in wound healing and currently, several research studies have been focused to treat this condition using scratch wound assay which is commonly performed to study the cell migration activity. Aloe vera is very aware of its wound healing property and Costus pictus is orally consumed to control the blood glucose levels. The present study is aimed at comparing the scratch closure rate of cells that were grown under optimum and increased glucose conditions when treated with the extracts of Aloe vera / Costus pictus. The aqueous extracts of Costus pictus and Aloe vera were prepared and characterized spectroscopically using Fourier Transform Infra-Red (FTIR) and UV-Vis spectroscopy. In vitro activities of both the plant extracts were revealed and the cytotoxicity of extracts on 3T3 fibroblast cells were tested by MTT assay. The treated 3T3 fibroblast cells were subjected to wound scratch assay under normal and increased glucose levels and the scratch closure rate was calculated at the intervals of 24, 48 and 72 hrs. The treated 3T3 fibroblast cells under increased glucose conditions exhibited a scratch closure rate of 99.09 % at 72 hours whereas that of the same cells grown under optimum glucose conditions displayed a scratch closure rate of 99.58 %.