Spectrophotometric Simultaneous Estimation of Raloxifene and Naringin by Vierordt’s Method

Abstract

A new Vierordt’s Method or simultaneous equation method was developed and validated for the simultaneous estimation of raloxifene hydrochloride (RAL) and naringin (NAR) in their pure binary mixture and nanoformulation dosage form. The method was based on measuring absorbance at two wavelengths 289nm and 284nm (λmax of RAL and NAR) respectively in methanol. Absorptivities of RAL at 289 and 284nm were (689.5 and 683.13), and those of NAR was (282.45 and 316.75). The % recovery values for accuracy studies were found to be in the range (102% and 96.45%) for RAL and NAR, respectively. The % recovery values for precision studies were found to be in the range (Intraday precision; 102.78% and 96.13%), ( Interday precision; 102.58% and 96.02%) for RAL and NAR, respectively were found to be satisfactory. The linearity of the calibration plots was confirmed by the high value of the correlation coefficients (R2 = 0.9994 for RAL and 0.995 for NAR). The limit of detection and limit of quantification were theoretically calculated which were found to be (0.57µg/ml and 0.613µg/ml) and (4.25µg/ml and 4.55µg/ml) for RAL and NAR, respectively. Robustness was also carried out and %RSD was found to be less than 2.0%. The proposed method was successfully used to estimate RAL and NAR in the pure binary mixture and their nanoformulation dosage forms.