Viability Test on Various Size Tooth Grafts in Cultured Fibroblasts (BHK 21)

Abstract

Periodontal tissue damage in the form of periodontitis can be treated by surgical and non-surgical. Phase augmentation surgery can be done by adding a graft to stimulate bone growth. One of the augmentation materials for the treatment of periodontal bone defects is a tooth graft. The required augmentation materials for use are non-toxic and biocompatible. This research aimed to determine the viability of tooth grafts against Baby Hamster Kidney 21(BHK-21) fibroblasts cell cultures. This experiment is an experimental laboratory study on cell cultures by post-only control group design. In this study, there are seven treatments: small size particle 0.2g; small size particle 0.4g; medium size particle 0.2g; medium size particle 0.4g; large size particle 0.2g; large size particle 0.4g. An MTT assay test was performed, and the absorbance was observed using the ELISA reader with a wavelength of 600nm. A one-way ANOVA test is performed to determine the difference between the treatment groups, using post hoc Tamhane’s T2 test because the data are not homogeneous. Different test results showed all values of p>0.05. This means that there are no significant differences between all groups. The tooth graft has high viability for the fibroblast cells.