Profile of RT-PCR for SARS-CoV-2: A Preliminary Study from COVID-19 patients in Government Medical College and Hospital, Doda, Jammu and Kashmir

Abstract

A new corona virus pandemic (COVID-19) has caused 7,010,568 deaths worldwide. The three stages of the Covid-19 disease course include the asymptomatic stage, upper respiratory tract infection, and lower respiratory tract infection/ARDS. Materials and methods: We examined the dynamical profile of SARS CoV-2 in 100 COVID-19-infected hospitalised patients from Jammu and Kashmir's Chenab valley (Doda, Bhaderwah, and Kishtwar). Patients' throat and nasal swab samples were taken at various times following the onset of symptoms. RT-PCR test with a COVID-19 nucleic acid detection was used to find SARS-CoV-2. The real-time RT-PCR assay examined two target genes: nucleocapsid protein (N) and open reading frame 1ab (ORF1ab). AIM: To assess the SARS-CoV2 positive detection rate using real-time polymerase chain reaction (RT-PCR). Results: At one week after the onset of symptoms, the PCR positivity rate was 100%; it then gradually decreased until it reached 100% negative results at six weeks following the onset of symptoms. Up to 6 weeks following the start of symptoms, viral shedding took place. For senior individuals, an extended monitoring period is required. Conclusion: This study discovered that individuals with extended viral nucleic acid conversion were typically older and had more co-morbid conditions, such as diabetes and hypertension.