Potential uses of Teki Grass (Cyperus rotundus L.) Tubers as Antioxidants in Diabetes Mellitus: In vitro Studies

Abstract

Diabetes Mellitus (DM) is a metabolic disorder characterized by hyperglycemia. The antioxidant activity in plants can be related to antidiabetic. Teki grass tuber (Cyperus rotundus L) is a part of a plant that contains flavanone compounds which have anticancer and antioxidant activity. Objective of this study was to investigate the potential of extracts and fractions of taki grass tuber as a source of natural antioxidants based on total quantities of phenolics, flavonoids, and antioxidant activity. Collect data in this study using qualitative and quantitative research methods by obtaining data obtained from the results of manufacturing simplicia, the extraction process using solvents with different concentrations, screening of phytochemicals for secondary metabolites, and measurement data from the treatment of test animals in the study. Results showed that the ethanol extract of teki grass tuber (EETG) contained alkaloids, tannins, terpenoids and saponins while the fraction of teki grass tuber contained alkaloids, tannins, terpenoid saponins and flavanoids. The total flavonoid content in the ethanol extract and ethanol fraction of teki grass tuber was 8.14 mg quercetin equivalent (QE)/g and 13.25 mg QE/g, respectively. The IC50 values of teki grass tuber ethanol extract and ethanol fraction with 5 times replications were 49.55 ± 0.0002 µg/ml and 43.18 ± 0.00088 µg/ml, respectively, indicating very significant antioxidant activity. The ethanol extract and ethanol fraction of teki grass (Cyperus rotundus L) tubers contain flavonoids and have antioxidant activity.