Evaluation of Nephroprotective effect of Ubiquinol on Ifosfamide Induced Nephrotoxicity in Albino Wistar Rats

Abstract

To determine nephroprotective activity of ubiquinol on ifosfamide induced renal damage by measuring the oxidative stress, biochemical parameters and histopathological examination. Thirty-six rats were divided into 6 groups, Group 1 rats were treated with 2ml of corn oil (vehicle) p.o., Group 2 and 3 received only ubiquinol of 10mg/kg/day and 50mg/kg/day respectively. Ubiquinol was prepared as oral suspensions with 2ml of corn oil. Group 4 received ifosfamide 80mg/kg/day, i.p. for 3 days. Groups 5 and 6 also received ubiquinol 10mg/kg/day and 50mg/kg/day respectively p.o. for 14 days. Then, we added ifosfamide to both the groups and dosed concomitantly along with ubiquinol from 8th -10th day. Ubiquinol proved to be an effective renoprotectant by decreasing the kidney to body weight ratio in ubiquinol treated IFO groups as compared to IFO group (p<0.05). Biochemical tests done on day 9 revealed that there was no significant difference in levels of Sr.Creatinine and total protein, but the levels of BUN were found to be significantly higher in IFO treated groups as compared to corn oil group (p<0.05). On day 15, it was seen that significant improvement in Sr.Creatinine, BUN and total protein levels of ubiquinol treated IFO groups as compared to IFO group (p<0.01). The levels of MDA and GSH were reversed in ubiquinol treated IFO groups as compared to IFO group (p<0.01). Histopathological examination supported that ubiquinol preserved the normal architecture of kidney in ubiquinol treated IFO groups. Ubiquinol proved to be an effective nephroprotective agent against ifosfamide induced nephrotoxicity.