Ameliorative effects of aqueous extract of Momordica charantia seed against Collagen induced Rheumatoid Arthritis in rats

Abstract

Rheumatoid Arthritis is one of the leading causes of disability and impairment ubiquitously which has worldwide prevalence of 1% population with increasing number of newer cases. RA is considered as an autoimmune disorder but systemic and chronic inflammation with metabolic factors leads extra organ manifestations with management also. The present study was designed to evaluate ameliorative effects of aqueous extract of Momordica charantia seed in wistar male rats, sensitized with bovine collagen type II (0.1ml sub planter) and compared with Methotrexate (0.6mg/kg i. p.) as standard treatment. The estimations were done on the basis of inflammatory components (Paw volume, CRP, ESR, IL-6 and TNF-α), Immune components (Arthritic score, Arthritic index, Anti-CCP) and Disease progression index (Disability, Radiological and histopathological examinations) for 42 days. Test groups of aqueous extracts of Momordica charantia seed (100mg/kg, 200mg/kg and 400mg/kg) were analyzed using statistical method (one way ANOVA and repeated measure ANOVA). On the basis of results preventive treatment of MCAE 400mg/kg p.o. proved to possess anti arthritic activities against inflammatory as well as immune components activated by collagen induced RA.