Investigations of Biological activities and Characterisation of Phytochemical constituents of Pulicaria laciniata extracts, an Endemic plant growing in south of Algeria

Author:

Bireche Kamilia1,Dendougui Hocine2,Gultekin-Ozguven Mine1,Hasni Sara3,Rahmani Yousef4,abid Asma2,Belgidoum Mahdi2,Hadjadj Mohamed2,Ozçelik Beraat5

Affiliation:

1. Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, Maslak, TR-34469, Istanbul, Turkey.

2. Kasdi Merbah University Ouargla, Faculty of Mathematics and Material Sciences, Department of Chemistry, Valorization and Promotion of Saharan Resources (VPRS) Laboratory, 30000 Ouargla, Algeria.

3. Kasdi Merbah University Ouargla, Natural Sciences and Life Sciences faculty, Biological Sciences Department, Lab of Protecting Ecosystems in Arid and Semi-Arid areas., Ouargla 30000, Algeria.

4. Center for Scientific and Technical Research in Physico-chemical Analysis (CRAPC), Ouargla, Algeria.

5. Bioactive Research & Innovation Food Manufac. Indust. Trade Ltd., Katar Street, Teknokent ARI-3, B110, Sarıyer, 34467, Istanbul, Turkey.

Abstract

Since limited research was conducted on studying the Pulicaria laciniata (P. laciniata (Coss and Kral)) aerial parts, this study was carried out. This study aimed to determine and classify three main extracts constituents by GC/MS, HPLC-PDA and screen there in vitro biological potency as the Antioxidant capacity, anti-diabetic, anti-Alzheimer's and anti-obesity activities. In which three extraction was affected with Chloroform, Ethyl acetate and n-Butanol each extract was characterised with GC/MS and HPLC-PDA, after that the capacities of the extracted molecules was evaluated against the ABTS free radical, ferric iron, α-amylase, α-glucosidase, acetylcholine esterase and the pancreatic lipase in order to investigate the mentioned activities. As a result, the n-Butanol and Ethyl acetate extracts had more polyphenols than the Chloroform extract. The most potent anti-obesity and anti- anti-alzeihmer action were demonstrated by the Chloroform extract through inhibition of pancreatic lipase and acetylcholinesterase.

Publisher

A and V Publications

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