Analysis of Total Flavonoid and Total Phenolic Content of Red Algae (Eucheuma denticulatum (Burman) Collins et Harvey) Extract and Tempeh Extract

Abstract

The red seaweed Euchema denticulatum (commonly referred to by the trade name Spinosum) is primarily farmed for extraction of carrageenan for application in foods. Tempe is a fermented soybean dish that is well-known for having a lot of protein. In the world, there are various ways to make tempeh, including soaking it in vinegar before fermentation. This study aims to determine the levels of total flavonoids and total phenolics in red algae and tempeh samples. Total phenolic content test was carried out using the Folin-Ciocalteu method using UV-Vis spectrophotometry at a wavelength of 760.5 nm. Total Flavonoid content test for total phenolic content was carried out quantitatively using a uv-vis spectrophotometer at a wavelength of 370 nm. The results showed that the average total flavonoid content in red algae was 55.25 mg/kg, and the total phenolic content in red algae was 420.56 mg/kg. whereas in tempeh the average total flavonoid content in red algae was 223.20 mg/kg, and total phenolic in tempe was 736.40 mg/kg.