Affiliation:
1. Department of Pharmaceutical Analysis, GITAM School of Pharmacy, Visakhapatnam, India.
Abstract
Trastuzumab and Pembrolizumab, are both integral to cancer treatment. This study focused on the development of a Reverse-phase high-performance liquid chromatography technique that has been validated for simultaneous assay of Trastuzumab and Pembrolizumab using Luna Phenyl Hexyl column with UV detection at 215 nm, and the run time is 5 minutes. The retention times for Trastuzumab 2.730 min and Pembrolizumab 4.468 min were determined. The method exhibited a concentration range of 110–660 μg/ml for Trastuzumab and 12.50–75 μg/ml for Pembrolizumab. The Limit of Detection and Limit of Quantification were determined to be 0.66μg/ml and 2μg/ml for Trastuzumab,0.075 μg/ml and 0.25 μg/ml for Pembrolizumab, respectively.
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