Naringi crenulata leaf extract alleviates Beta Amyloid Protein Induced Alzheimers disease in mice

Abstract

Oxidative stress is involved in Alzheimers disease(AD)-type neurodegeneration with cognitive impairment, as well as age-related cognitive loss. The purpose of this study was to investigate the effects of Naringi crenulata leaf extract (NCLE) on cognitive dysfunction, oxidative stress in the hippocampus in a mouse model of beta-amyloid-induced AD. The animals were given NCLE at two different doses (50mg/kg and 100mg/kg, orally) for 21 days. On the day 15th receiving intracerebroventricular-amyloid beta peptide25-35 injection (3mg/kg; single dose). Y maze and morris water maze paradigms were used to examine behavioural changes on the day 22. On the 22nd day, mice were sacrificed to measure malonaldehyde (MDA), myeloperoxidase (MPO), nitric oxide (NO), reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) in mouse brain tissues. The enhancement of habituation memory and the step-down inhibitory memory in the experimental animals. Pro-inflammatory cytokines levels were decreased and anti-inflammatory cytokines were enhanced. The study implies that NCLE can protect against cognitive impairments and oxidative stress caused by amyloid beta, and that it could be employed as a adjunct therapy in the treatment of neurodegenerative illnesses such as Alzheimers.