Estimation of Phenolic acid and Flavonoids in leaves of Amaranthus viridis and Amaranthus spinosus

Abstract

Objective: To explore physiochemical and phytochemical standardization of leaves of Amaranthus viridis and Amaranthus spinosus. Method: As per WHO guidelines, physical standardization parameters with various solvents system were evaluated. The preliminary phytochemical screenings were adopted for disclosing the existence of phytochemicals in the discrete extracts. Thin layer chromatography and HPTLC were employed for methanolic extract of leaves of Amaranthus viridis and Amaranthus spinosus. Result: Preliminary phytochemical screening with various extracts reveals phytoconstituents. HPTLC fingerprint were executed for leaves of Amaranthus viridis and Amaranthus spinosus using selected solvent system. HPTLC analysis implies the presence of phenolic compounds and flavonoids in both the plant Amaranthus viridis and Amaranthus spinosus. Conclusion: Quercetin and Gallic acid was raise to be more in Amaranthus viridis compared to Amaranthus spinosus, however rutin was reported only in Amaranthus spinosus. The outcome of the research leads for isolation, purification and utilization in herbal industries.