Estimation of Ceftazidime and Avibactam in their Bulk and Formulations by a newly Developed and Validated of Stability Indicating RP-UPLC Method

Abstract

A delicate, fast, Accurate, exact and steadiness, showing isocratic RP-UPLC method was developed for the concurrent assurance of the Ceftazidime and Avibactam in bulk and formulation. To optimize, a column HSS C18 100 x 2.1mm, 1.8µm, Mobile phase containing water: acetonitrile choose in the ratio 75:25v/v was pumped through column at a flow rate of 0.3ml/min at 260nm, initiate to be an efficient method for elution of drug with good peak shapes as well as retention times. Rt of Avibactam and Ceftazidime were initiate to be 1.463 min and 2.109 min. %Recovery was obtained as 100.07% and 100.08% for Avibactam and Ceftazidime separately. LOD, LOQ values got from relapse conditions of Avibactam and Ceftazidime were 0.85, 2.56 and 3.53, 10.70 correspondingly. Regression equation of Ceftazidime is y = 7883.2x + 12277, and y = 3279.1x + 1137 of Avibactam.