Anti-Urolithiatic Activity of the Ethanolic Extract of Cassia auriculata against Ethylene Glycol Induced Urolithiasis in Experimental Rats

Abstract

The objective was to investigate the anti-urolithiatic effect of ethanolic extract of Cassia auriculate on ethylene glycol (EG) induced urolithiasis in experimental rats. The animals were divided into five groups of six animals each. Except the normal group all the other groups received ethylene glycol 0.75% and Ammonium chloride 1% v/v in water induce orally for 28 days. Normal groups received plain water orally. The standard group received cystone 750mg/kg b.w orally. Test groups received EECA 200mg/kg and 400mg/kg b.w orally. On the 28th day, blood and urine samples were collected and used for estimation of biochemical parameters such as calcium, phosphates, oxalates, creatinine, uric acid, SOD, and CAT followed by histopathological studies. Treatment with EECA was found to exert dose dependent anti urolithiatic action. Increased urine volume in EECA treated groups as compared to diseased group was indicative of diuretic property. Elevated calcium, phosphate and oxalate levels in diseased group animals were found to be decreased in animals treated with EECA. Increased levels of serum calcium, creatinine and uric acid were considerably brought down towards normal values in proportion to EECA doses administered. Antioxidant parameters like SOD and CAT were decreased along with significant increase in MDA levels which is the main product of lipid peroxidation in EG treated rats. However the rats treated with EECA showed significant improvement in these parameters. Hence it is concluded that the ethanolic extract of Cassia auriculata possess anti-urolithiatic activity.