Protective effect of Chrysin as Antioxidant, Anti-inflammatory and Anti apoptotic agent in Transient global cerebral Ischemia - Reperfusion injury among diabetic rats

Abstract

Aim: Oxidative stress, inflammation and apoptosis have been implicated as pathological conditions in ischemic stroke, further these conditions worsen in the presence of diabetes. This study was designed to investigate whether the chrysin has protective role against ischemia reperfusion injury through anti-oxidant, anti-inflammatory and anti-apoptosis mechanisms in diabetic rats. Methods: Streptozocine (50mg/kg) intraperitonially given at once to induce the diabetes. Common carotid artery occlusion (30 min) and reperfusion (4 hr) was employed to induce cerebral infarction in diabetic rats. The animals were divided in to groups as: normal, sham, ischemia-reperfusion and chrysin treated (10, 20, 30 and 40mg/kg). These were used for estimation of percentage of cerebral infarction. 30mg/kg dose was used for further estimation of inflammatory markers like TNF-α, IL-6, IL-10 and oxidative stress markers (xanthine oxidase, glutathione, NADPH, malondialdehyde, superoxide dismutase and catalase), apotosis markers (Bcl2, BAX and Hsp90) were estimated and also glutamate and aspartate levels were measured in brain tissues. Results: Dose dependent reduction in percentage of cerebral infarction was observed in chrysin treated groups. With chyrsin 30mg/kg the apoptosis, inflammatory and oxidative stress markers like BAX, Hsp90, TNF-α, IL-6, myeloperoxidase, malondialdehyde, NADPH and xanthine oxidase levels were reduced and there was a significant increase in anti-apoptosis, anti-inflammatory and anti-oxidant markers like Bcl2, IL-10 levels and glutathione, catalase, superoxide dismutase which was also accompanied with elevated glutamate and aspartate levels. Conclusion: Collectively, these results show that chrysin possess a cerebroprotective effect against ischemic reperfusion injury in the diabetic rats.