UV Spectrophotometric Quantification of Niacinamide in Pharmaceutical Dosage Form by Multivariate Calibration Technique

Abstract

The quantification of Niacinamide in pharmaceutical dosage form employing Multivariate Calibration Technique is detailed in the current study. The λmax of Niacinamide was found to be 262 nm. The linearity of the developed method was accessed at five different concentrations ranging from of 8-24µg/mL. Correlation coefficient (r2) value was found to be above 0.998. The repeatability of the method was predicted by performing Intraday and Interday precision studies. The developed method was validated according to ICH guidelines and results proved that the developed method was simple, linear, precise, accurate and sensitive. The developed and validated Multivariate Calibration Technique can be further employed and released into QC for the quantification of Niacinamide in pharmaceutical dosage form.