Solubility Studies and Validation of Lovastatin using High Performance Liquid Chromatography Method

Abstract

This study aimed to determine the solubility of lovastatin (LV) in different oil, surfactant, and co-surfactant using the high-performance liquid chromatography method. LV was solubility studies in different vehicle. The different vehicle used almond oil, sunflower oil, oleic acid, olive oil, soybean oil, and corn oil, isoprophyl myristate, myoglyol, tween 80, tween 20, and cremophor R.H. 40, propylene glycol, and PEG 400. Each of them was added lovastatin until saturated. The mixtures were mixing, sonicating, putting in the water bath and standing for 24 hours, then centrifugated. Each of the aliquot 2 µL diluted with acetonitrile and determination of concentration lovastatin using HPLC, with detector ultraviolet at 237 nm. Before determinate LV validated, and curve calibration at range 2-16 µg/mL was made. This study using the HPLC method with detector UV 237 nm, Agilent C 18 (4.6 x 150 mm 5 µ) column, and acetonitrile: water (70:30 v/v) as mobile phase. Calibration curve of lovastatin at the range 2-16 µg/mL with linear regression 0.999. Accuracy and precision showed that. Lovastatin has high soluble in oleic acid, tween 80, and PEG 400.