Immunological and Enzymatic study of Staphylococcus aureus Bacteria and fungi isolated from oral cavity

Abstract

The current work aimed to study the propagation of Staphylococcus aureus and Candida sp. and. in oral cavity of the human, and their effective on innetimmune response and to describe the biochemical &kinetic properties of PLB from the Staphylococcus aureus. and asporogenous yeast C. tropicalis. The study included 25 patients, ages (12-65) years, with periodontal diseases and at least two sites with probing depth ≥ 3 mm. Participants' data were assessed individually. A sample is taken from the oral cavity (mucous membrane, tongue and cheek) with a sterile swab. Among all the patients studied, (16) 64% showed Staphylococcus in the oral cavity while Streptococcus sp (3) 12% showed candidiasis in the oral cavity and (5) 20 % of other bacteria(1) 4% in the oral cavity. The effect of microbial inflammation on the multidirectional secretion of interleukin (IL) -10, IL-6 has been studied in patients with oral cavity infections. Samples and wash swabs were taken from 25 healthy patients and 25 patients. The supernatant fractions of IL-6 and IL-10 were examined by commercial ELISAs. Analysis of interleukin levels in the two groups with the Mann-Whitney test.IL-6 levels various significantly between the patients, the control group, and the control groups. Median levels of IL-10 in both groups were uniformly low but the mean levels were not statistically different. The observed change in bacterial flora that is characteristic of stomatitis does not enhance or suppress oral levels of IL-6 and IL-10. Staphylococcus aureus, and Candida tropicalis have been isolated oral cavity and under the periodontal sinuses infection. It was filtered 2.5 fold at40 ºC from a culture broth and has an estimated molecular mass of 50kDa.The enzyme was two pH targets, one acid (pH 4.0) and other alkaline (pH 7.5). At acidic pH the enzyme showed a higher affinity for all tested phospholipids without metal ions while its affinity affected by the presence of metal ions at alkaline conditions and the enzyme activity inhibited by Fe3+ and Hg2+.