Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line-Reference-Cited by-同舟云学术

Cytotoxic effects of Chloroform-methanol extract, protein extract and various fractions of ethanolic extract of Curcuma amada rhizome on HCT116 colon cancer cell line

Published:2022-11-24 Issue: Volume: Page:4955-4958
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

S. Patil Vaibhav.¹,B S Varashree²,Ranganath Pai K. Sreedhara³,Hari Gangadhar⁴,Priya Keerthi⁴

Affiliation:

1. Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher education (MAHE), Manipal 576104, Karnataka, India.

2. Professor and Head, Department of Biochemistry, Kasturba Medical College, Manipal, Manipal Academy of Higher education (MAHE), Manipal 576104, Karnataka, India.

3. Professor and Head, Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal-576104, Karnataka, India.

4. Research Scholar, Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), Manipal-576104, Karnataka, India.

Abstract

Cytotoxic effects of chloroform-methanol extract, protein extract and various fractions of ethanolic extracts of Curcuma amada rhizome were investigated on Human colorectal cancer cell line (HCT116). The ethanolic extract showed excellent cytotoxicity of all extracts with an IC50 value of 14.46µg followed by the Chloroform-methanol extract with an IC50 value of 18.31µg. The various fractions of ethanolic extracts fractionated with petroleum ether, ethyl acetate, n-butanol were also tested for cytotoxicity independently. Ethyl acetate fraction gave an IC50 value of 1012µg and n-butanol IC50 value was 43.19µg, with petroleum ether fraction giving no cytotoxicity. The crude proteins isolated from the rhizome showed good cytotoxicity and gave an IC50 of 43.1µg.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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