Affiliation:
1. D.S.T.S. Mandal’s College of Pharmacy, Solapur.
Abstract
RP-HPLC method has been developed and validated for simultaneous estimation of Tobramycin and Dexamethasone in eye drop. The chromatographic separation was carried out isocratically on Agilent Zorbax SB Aqueous C-18 column (250x4.6mm, 5µm) using a 0.1% Formic Acid Water: Acetonitrile (60:40% v/v) as mobile phase at flow rate of 1ml/min and detected by using photo diode array detector at 210nm. The retention times were 1.9 and 4.2 min for tobramycin and dexamethasone respectively. Linearity was obtained in the range of 240-360µg/ml and 80-120µg/ml for tobramycin and dexamethasone respectively. The developed method was validated according to ICH guidelines for specificity, linearity, range, accuracy, precision, system suitability parameters. The proposed method was found to be specific, accurate and precise. This method can be employed for routine analysis of simultaneous estimation of tobramycin and dexamethasone in eye drop.
Subject
Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Reference16 articles.
1. Henry FC. Antimicrobial agents: The Aminoglycosides. In Goodman and Gilman’s. The pharmacological basis of therapeutics. Edited by Hardman JG and Limbird LE. McGraw-Hill, New Yark. 2001; 10th ed: pp. 1209-1233.
2. Ruckmani et al. A simple and rapid high-performance liquid chromatographic method for determining Tobramycin in pharmaceutical formulations by direct UV detection. Pharma Methods. 2011;2(2): 117-23.
3. Nagaraju et al. Novel stability indicating RP-HPLC method for the simultaneous estimation of Tobramycin and Loteprednol in pharmaceutical dosage forms. GSC Biological and Pharmaceutical Sciences. 2020; 10(1):73-80.
4. Jagan R, Ganpatay S. A Validated Stability Indicating RP-HPLC Method for Simultaneous Estimation of Tobramycin and Ceftazidime in Pharmaceutical Formulation. International Journal of Pharmacy. 2015; 5(3):976-984.
5. Tobramycin [Internet] Drug bank [cited 2020 Oct 27 ]. Available from: https://go.drugbank.com/drugs/DB00684
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献