Screening of Potential plants from Kalimantan as an Antimicrobial agent for Coliform bacteria

Abstract

The use of extract plants as a therapy method for bacterial illnesses is possible. Plants have antibacterial and antioxidant properties and include various chemicals that protect the human body from infections. And coliform bacteria are a major cause of public health issues. This study aimed to determine the potential of a variety of plants as an alternative antibacterial agent. The study was carried out with extract plants utilizing the disc diffusion method. The ultrasonic-assisted extraction procedure with n-hexane, DMSO and methanol to extract the leaf and stem bark of the plants. Zones of inhibition are measured using the Disc Diffusion Method, and the diameter of bacterial growth inhibition is measured in millimeters. At a 10 mg/mL dosage, all plant extracts had a growth-inhibitory impact against at least one of the three microorganisms tested, especially Enterobacter aerogenes. The extract of Luvunga scandens leaf had the strongest antibacterial action among the ten active plant extracts tested inhibiting the growth of Escherichia coli, Klebsiella pneumonia, and Enterobacter aerogenes at MICs of 10 mg/mL. It was concluded that all plants tested had potential as an antibacterial against coliform bacteria.