In vitro Antioxidant Potential and Anti-bacterial Activities of Rajanyadi Churna Extracts Against Escherichia coli

Abstract

Rajanyadi churna (RC) is a polyherbal traditional formulation prescribed in Ayurveda for treating diarrhea, gastric disorders, asthma, fever, anemia and jaundice. In the current experiment, various in vitro models of antioxidant activity, and antibacterial activitity against Escherichia coli of Rajanyadi churna alcoholic extract (RCAL) and Rajanyadi churna aqueous extract (RCAQ) are presented. In vitro antioxidant potential was determined for both the extracts in various models like ABTS, Nitric Oxide, DPPH, Superoxide and Hydrogen peroxide scavenging assays. The minimum inhibitory concentration (MIC) of RC extracts against Escherichia coli, Gram negative bacteria were determined using the micro-dilution technique (ATCC 11775). Our study showed a remarkable improvement in the antioxidant activity of RCAL and RCAQ in all the in vitro models. The percentage of inhibition for RCAL and RCAQ were found to be 31% and 25% respectively against E. coli. From this experimental study, we conclude that Rajanyadi churna extracts showed good antioxidant activity and antibacterial activity against E. coli.