Extractive determination study of Etravirine by using Tpooo as an Analytical reagent in Pure and Pharmaceutical dosage forms

Abstract

This study developed a sensitive and straightforward extractive spectroscopic method to estimate Etravirine (ETR) using TPooo as an analytical reagent in pure and pharmaceutical dosage forms. This method was achieved based on the extractable chloroform complex formed with Tropeoline ooo (TPooo) in an acidic media. Following Beer’s law, the extractable complex showed the absorbance maximum at 485nm at the concentration ranges between 12.5-75 µg/ml with the molar absorptivity 2.195 x 103 L/mole /cm and the Sandell’s sensitivity 0.1549 µg cm-2. The result of Etravirine estimation for the present method has been validated statistically by recovery studies, and the developed method was simple, sensitive, accurate, and precise. It was validated following International Conference on Harmonization (ICH) guidelines and also successfully applied for the estimation of Etravirine in tablet dosage forms.