Development and Validation of a RP-UHPLC Method for estimation of Gliclazide loaded Microsphere-Reference-Cited by-同舟云学术

Development and Validation of a RP-UHPLC Method for estimation of Gliclazide loaded Microsphere

Published:2022-02-26 Issue: Volume: Page:773-778
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Ahmed Abdul Baquee¹,Bordoloi Ripunjoy²,Bhattacharya Kunal³

Affiliation:

1. Girijananda Chowdhury Institute of Pharmaceutical Science, Tezpur, Assam – 784501.

2. Assam Science and Technology University, Jalukbari, Guwahati, Assam-781013.

3. NETES Institute of Pharmaceutical Science, Mirza, Kamrup, Assam-781125.

Abstract

Objective: The objective of the current work is to develop and validate a simple yet effective, efficient and reproducible RP-UHPLC method for the determination of Gilcazide loaded in a microsphere formulation. Methods: The chromatographic analysis was carried out on Thermo Scientific (Dionex Ultra 3000plus) UHPLC using a stainless steel YMC C8 column (25cm × 4mm) packed with endcapped octylsilane bonded to porous silica (4µm) in isocratic elution mode using phosphate buffer pH 3.4 and HPLC grade acetonitrile in the ratio of 20: 80 (v/v) as eluent. The flow rate was adjusted as 1ml/min and eluent was detected at 230 nm. The retention time was observed at 1.9 min. Results: The linear dynamic range for the developed UHPLC method was found in the concentration range of 2-10µg/ml and percentage recovery was in the range of 94%-98%. Drug Entrapment Efficiency Data of Gliclazide loaded microsphere was found to be 93.72% Conclusion: In this RP–UHPLC method, the linearity was within the range of 2–10μg/mL and the validation parameters were within the limits was performed as per ICH guidelines. The % Drug entrapment Efficiency of the Gliclazide loaded microsphere was found to be 93.72 % which was satisfactory.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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