The Viability of Collagen Peptide from Osphronemus goramy Fish Scale Extract on Human Gingival Fibroblast

Abstract

Background: Damage to the periodontium tissue requires a regenerative treatment to increase the dimensions of the lost bone. This research was conducted to find another regenerative material with the use of type 1 collagen peptides derived from gourami fish scales. This study was conducted to test the viability of gourami scales collagen peptides on Human Gingival Fibroblast cells for 24 hours. Purpose: To determine the viability of Human Gingival Fibroblasts (HGF) after the administration of collagen peptides of gourami fish scales. Method: HGF was taken from healthy gingiva and planted in 96 well plates. The type 1 collagen peptides of gourami fish scales with concentrations of 0.32 mg / ml, 0.16 mg / ml, 0.04 mg / ml, 0.02 mg / ml and 0.01 mg / ml were inserted into each well and incubated for 24 hours. MTT Assay was performed to see the viability of fibroblast cells. Results: There was an increase in the viability value from a concentration of 0.32 mg/ml to 0.01 mg/ml. The concentration of 0.01 mg/ml showed the highest viability. Conclusion: The collagen peptide is a potential substance for tissue engineering. The concentration of 0.01 mg/ml collagen peptides shows the highest HGF viability.