Development and Validation of RP-HPLC Method for the Estimation of Alogliptin in API And Tablet Formulation-Reference-Cited by-同舟云学术

Development and Validation of RP-HPLC Method for the Estimation of Alogliptin in API And Tablet Formulation

Published:2022-04-23 Issue: Volume: Page:1791-1794
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Ingle Suyash¹,Tegeli Varsha¹,Birajdar Avinash¹,Nangare Gajanand¹

Affiliation:

1. D.S.T.S. Mandal’s College of Pharmacy, Solapur - 413004, Maharashtra, India.

Abstract

The analytical method was developed and validated for determination of Alogliptin in bulk and pharmaceutical dosage forms by High performance liquid chromatography. The separation was carried out on Zorbax SB-Aq (250 x4.6mm,5µ ID) column. The mobile phase consists of 0.1% TFA Water : ACN in the ratio 62:38 at flow rate 1ml/min with diode array detector wavelength at 290nm.The column temperature was adjusted at 30º ± 0.5°C with injection volume 10µl.The retention time of Alogliptin was 3.06min. The linearity of the calibration curve was linear over the concentration range 25-75µg/ml (r2=1). The validation was carried out as per ICH guidelines. The development of method was easy, rapid, linear, precise, accurate and consistent.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

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