HPTLC Finger print Analysis and In vitro Cytotoxicity Study against A549 cell line for Methanol and Chloroform extract of bark of Erythrina stricta Roxb.

Abstract

Plant provides various important phytoconstituents in the form of primary and secondary metabolites. Secondary metabolites obtained from the plants possesses significant biological activities. The plant phytochemicals are useful for scavenging free radicals and also in the treatment of cell injury. The proper identification and authentication of the plant secondary metabolites is important for quality control purpose. There are various chromatographic tools like HPTLC, HPLC and GC are interest of researcher for carrying out the authentication of the plant secondary metabolites. Among these, HPTLC is used widely for the plant authentication for its fingerprint ability for plant constituents. In this study, methanol (ESM) and chloroform (ESC) extracts of Bark of Erythrina stricta Roxb. were selected for its HPTLC fingerprint profile and In vitro cytotoxicity study for A549 cell lines (lung cancer). Plants were collected from in and around of Aizawl city and authenticated from BSI, Shillong. Bark of plants were prepared and extracted using soxhlet extractor with different solvents gradually increasing their polarities (Petroleum ether, chloroform and methanol). Solvent systems for chromatography were developed and HPTLC fingerprint was carried out. MTT assay for cytotoxicity were performed against standard doxorubicin and IC50 concentrations were calculated. The HPTLC fingerprint showed the presence of various phytochemicals in chloroform and methanol extract. Cytotoxicity study suggested that the plants extracts reduce viable cells by exerting cytotoxic effect. These studies can be used further for exploration of other pharmacological actions including anticancer activity.