Cytotoxicity and Promising Anti-Biofilm of Curcuma Silver Nanoparticles against Candida albicans

Abstract

The fungal pathogens considered the major human opportunistic, 50–60% of candidiasis cases patients Candida albicans. Green synthesis of AgNPs from NaNO3 using Curcuma extract was performed. UV–Vis spectrophotometry and TEM characterization were measured. AgNPs absorption peak showed between 420-440 nm and results of TEM observed, the AgNPs has a uniform regular coccus shape and size of AgNPs was ranging from 50 to 12nm with average 37nm. The activity of AgNPs as antifungal agent against Candida albicans was determined using agar well diffusion method. AgNPs showed greater antifungal activity with inhibition zone diameter 28mm at 30μg/ml. The antibiofilm activity of AgNPs was assayed in 96-well polystyrene plates and 3 ml polyethylene tube. Obviously, remarkable progressive inhibition of biofilm formation was noticed with increasing of AgNPs concentrations. Determination of AgNPs cytotoxicity on normal lung fibroblast (WI-38) cells using MTT protocol was performed. The viability observed at concentration 31.25 was 44% with IC50 value 30.5μg ml−1. When Curcuma AgNPs are used to local infected wound, may inhibit Candida cells without bad side effect on host cells surrounding the infected wounds.