In- vitro Antiurolithiatic potential of leaves of Anneslea fragrans wall. against Calcium oxalate kidney stones and its FT- IR analysis

Abstract

Anneslea fragrans Wall. is an evergreen shrub or small tree that belongs to family Theaceae and can be characteristically identify by its unique aroma. A. fragrans has collected from Manipur, India and its leaves were extracted in methanol using soxhlet method. In- vitro antiurolithiatic potential has determined by turbidity changes in artificial urine method, nucleation assay, and aggregation assay. FT-IR has been used to study the functional group characterization of the leaves of A. fragrans. A. fragrans has showed significant in- vitro antiurolithiatic activity through turbidity changes in artificial urine, nucleation, and aggregation assay with maximum % inhibition at 1000µg/ml i.e. 78.1%, 32.32%, and 57.11% respectively. FT-IR analysis reveals the major functional groups including hydroxyl, saturated aliphatic, quinone or conjugated ketone and aliphatic flouro group. A. fragrans have shown excellent in- vitro antiurolithiatic activity and hence, can be consider as an effective herbal alternative or constituent of herbal formulation for treatment of urolithiasis.