Extract of Gigartina sp for Antibacterial Activities on Escherichia coli and Staphylococcus sp.

Abstract

Gigartina sp. was one of the red algae used as natural drugs due to its bioactive compounds i.e. acrylic acid, alkaloid compound, and phenol acting as antimicrobials. One of the methods to conduct antimicrobial isolation was extraction by maceration using solvent. Antimicrobial compound could provide various effects i.e. bacteriostatic, bacteriolytic, and bactericidal. Antimicrobial activities were measured by applying Minimum Barrier Concentration method. The research aimed to investigate antibacterial activities of Gigartina sp. algae extract, to find the optimum Gigartina sp. algae extracts to impede the growth of the tested microbes, to make a comparison between the antibacterial activities of Gigartina sp. algae extracts and that of antibiotic types, and to investigate the effects of the antibacterial characteristics of Gigartina sp on the growth of the tested microbes. Antibacterial activities were tested using agar dilution and liquid dilution with 0, 10, 15, 20, and 25mg/ml content. Antibacterial characteristics were found by measuring the total number of cells and the number of living cells in Gigartina sp. extract culture. Gigartina sp. algae extract with ethanol extracts gave a stronger effect to antimicrobial activities of S. aureus and E. coli than that with hexane and aqueous extracts. Areas of barrier zone on E. coli and S. aureus were 0.194cm2 and 0.378cm2 respectively. Gigartina sp. algae extract with ethanol extract on E. coli indicated activities similar to that of streptomycin and showed a significantly similar result between penicillin and streptomycin, so did Gigartina sp. alage extract on S. Aureus. The type of antimicrobial compound restriction of Gigartina sp. algae was bacteriolytic.