In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb.

Abstract

Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against α-amylase and α-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both α-amylase and α-glucosidase.