Viability of Mesenchymal Stem Cells from Stromal Vascular Fraction to Tissue Engineering Scaffold Collagen, Calcium Alginate, Oxidized Cellulose, Gelatin, And Amnion Membrane (Primary Cell Culture in Vitro Study)

Abstract

Introduction: Soft tissue engineering strategies targeting volume loss restoration has been developed as the current management of soft tissue defects. Important components of this method are cell sources, scaffold, and bioreactor. Adipose-derived mesenchymal stem cell (ADMSC) from a stromal vascular fraction (SVF) attached to scaffold and differentiated into defect tissue through cell communication. This study was aimed to investigate the viability of ADMSC from SVF combined with collagen, calcium alginate, oxidized cellulose, gelatin, and amnion membrane in vitro. Method: This true experimental done in the laboratory using 24 samples of SVF ADMSC that had been cryopreserved for 5 months and already get through the thawing process. Result: Based on the descriptive analysis with 4 repetition each group and control, gelatin shows average 90.928 and deviation standard 1,053, oxidized cellulose average 19,528 and deviation standard 2,543, collagen average 93,273 and deviation standard 1,195, calcium alginate average 92,953 and deviation standard 1,257, amnion membrane average 92,068 and deviation standard 2,467, control 95,995 and deviation standard 0,428. Conclusion: The use of gelatin, collagen, calcium alginate, and amnion membrane as scaffold show high viability results of ADMSC from SVF. Otherwise, oxidized cellulose scaffold has the lowest amount of viable cell. However, the most recommended scaffold material is amnion membrane because of the easiness of the production process, high availability, high potency of cell growth, and low cost.