Polyherbal effect between Phyllanthus urinaria and Curcuma longa as an Anticancer and Antioxidant

Abstract

A combination of herbals medicines is an alternative treatment choice for developing anticancer therapy because of its benefits, active compounds, and non-toxic side effect. This study investigates the anticancer and antioxidant activity of Phyllanthus urinaria and Curcuma longa water extract combination. The analysis of their bio-active components was done using LC-HRMS. The biological activity prediction was made using PassOnline and Cytoscape. Anticancer activity, apoptosis, and cell cycle assay were tested on treated T47D breast cancer cells line with the combination herbal extract and individual herbal extract compare to the untreated and cisplatin-treated control cells. This study also determined the antioxidant activity and phenolic and flavonoid total assay. The result showed that these herbs' compounds were predicted to have a biological function in cancer treatment. The herbal combination has efficiency inducing apoptosis with more than 50% compared to the individual herbal treatment and untreated control cells. Cell cycle analysis shows these herbals affect cell cycle arrest at G2/M phase compared to the control cells. The antioxidant activity and total phenol and flavonoid of P. urinaria and C. longa combination positively correlate with the anticancer activity result. Compusyn test results on cell toxicity parameters showed that the combination of both herbs had an additive effect (C = 1). Although this combination has no synergism effect, the water extract of P. urinaria and C. longa combination can be an anticancer agent.