Fourier Transform Infrared Spectrophotometry: An Eco-friendly green tool for quantification of Omeprazole in Pharmaceutical formulation

Abstract

An eco-friendly method for quantification of Omeprazole in Pharmaceutical solid dosage form has been developed using Fourier transform infrared spectrophotometry. The proposed method avoids use of solvents which are commonly used for other methods of quantification e.g. Liquid Chromatography, UV spectrophotometry etc. The developed method has been validated for quantification of Omeprazole in marketed formulation as per ICH Q2 R (1) guidelines. The method is based on Beer-lamberts’ law. For the quantification C-O stretch absorption band at 1204 cm−1 was selected which is typically in the range of 1300 – 1000cm-1 in IR spectrum. The linearity of Omeprazole was obtained in the concentration range of 5 – 30mg/g with R2 value of 0.999. The % recovery was calculated with Intra and Inter day precision study.