In vitro assay of ACE Inhibitor, Antidiabetic and Antioxidants activities from Indonesia Traditional Medicine (Jamu)

Abstract

Hypertension in people with diabetes causes accelerated complications of the heart and kidneys. Angiotensin Converting Enzyme (ACE) inhibitors are the first choice managing of hypertension in patients with diabetes mellitus. Herbal medicine is a native Indonesian treatment that has been used since time immemorial to overcome various diseases, including hypertension and diabetes mellitus. This study aims to standardize and assay the in vitro ACE inhibitor, antidiabetic, and antioxidant activities on Indonesian medicinal plants (jamu). Methods: Jamu was extracted by maceration using ethanol. Standardization includes non-specific and specific parameters. The activity of angiotensin-converting enzyme inhibitor, alpha-glucosidase enzyme inhibiting activity, and antioxidant activity using a spectrophotometer. The results showed that jamu met the requirements of both specific and non-specific parameters, had angiotensin-converting enzyme inhibitors, alpha-glucosidase enzyme inhibitors, and antioxidants activity with IC50 values 103.75 µg/ml, 49.95 µg/ml, and 11.4 µg/ml. Conclusion: jamu meets the requirements according to the Indonesian Herbal Pharmacopoeia standards and has antihypertensive, antidiabetic, and antioxidant activities.