Study on conditions for Gama buryric acid (GABA) production in Lactobacillus fermentum A01 isolated from human-Reference-Cited by-同舟云学术

Study on conditions for Gama buryric acid (GABA) production in Lactobacillus fermentum A01 isolated from human

Published:2021-04-29 Issue: Volume: Page:2188-2190
ISSN:0974-360X
Container-title:Research Journal of Pharmacy and Technology
language:en
Short-container-title:RJPT

Author:

Thien Phuc Cat¹,Thanh Vinh Doan Thi²,Khoa My Truong¹,Thu Hien Tran Thi²,Anh Dung Nguyen³,Minh Thu Tran Thi¹,Khue Nguyen Tu Hoang²

Affiliation:

1. School of Biotechnology, International University, Vietnam.

2. Vietnam National University – Ho Chi Minh City, Vietnam.

3. Faculty of Nautral Sciences, Thu Dau Mot University, Vietnam.

Abstract

Lactic acid bacteria play a vital role in biosynthesis of γ -aminobutyric acid (GABA) in the presence of glutamic acid - major substrate for the process. In recent study, Lactobacillus fermentum A01 (L. fermentum A01) isolated from various sources in Vietnam were screened for bacteria strains with high efficiency in GABA formation. L. fermentum was cultured in MRS broth containing 25 mg/mL monosodium glutamate (MSG), at pH of 6.5 and incubated at the optimal conditions (37ºC, for 24, 48, 72 h). After extraction and thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis, L. fermentum A01 showed the GABA yield about 1.34 mg/g in dried supernatant, suggesting L. fermentum A01 to be a promising GABA producer for food and pharmaceutical applications.

Publisher

A and V Publications

Subject

Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

Reference11 articles.

1. Dhakal R, Bajpai VK and Baek KH. Production of gaba (γ- Aminobutyric acid) by microorganisms: a review. Brazilian Journal of Microbiology. 2012; 43(4): 1230-1241.

2. Shi F and Li Y. Synthesis of γ-aminobutyric acid by expressing Lactobacillus brevis-derived glutamate decarboxylase in the Corynebacterium glutamicum strain ATCC 13032. Biotechnology Letter. 2011; 33(12): 2469-2474.

3. Schuller HM, Al-Wadei HA and Majidi M. GABA B receptor is a novel drug target for pancreatic cancer. Cancer. 2008; 112(4): 767-778.

4. Li H, Qiu T, Huang G and Cao Y. Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentation. Microbial Cell Factactories. 2010;9:85. Published 2010 Nov 12. doi:10.1186/1475-2859-9-85.

5. Park KB and Oh SH. Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Bioresource Technology. 2007; 98(8): 1675-1679.

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