Laboratory diagnosis of Yersinia pseudotuberculosis and Yersinia enterocolitica

Abstract

Enteropathogenic species Yersinia pseudotuberculosis and Yersinia enterocolitica are of great importance worldwide due to their ability to survive and preserve pathogenic properties in biotic and abiotic environments. They can cause sporadic and flare-up diseases. Therefore, it is extremely important to use modern methods of diagnosing diseases caused by these pathogens. The article analyzes the methods of laboratory diagnostics of enteropathogenic Yersinia (Y. pseudotuberculosis and Y. enterocolitica), including bacteriological, serological and molecular genetic. Modern data on the use of the polymerase chain reaction method, mass spectrometry (МALDI-TOF MS), dot immunoassay are presented. The scheme of the optimal protocol for the detection and identification of Yersinia is given. The combination of classical microbiological and molecular genetic methods quickly determines the presence or absence of a pathogen in a sample, which allows the bacteriological study to be purposefully used to verify the clinical diagnosis, confirm transmission factors and plan anti-epidemic measures.