Experimental study of the effectiveness of 6-methyl-3-(thietane-3-yl)uracil-containing eye ­ointment in the treatment of corneal thermal burns

Abstract

Aim. To evaluate the effectiveness of wound healing eye ointment with 6-methyl-3- (thietane-3-yl) uracil in the treatment of corneal thermal burn in the experiment. Methods. The study was conducted on 6 rabbits of the chinchilla breed. The results of treatment and histomorphological studies of the rabbits’ cornea are presented after experimental thermal burn by instillation of water with a temperature of 80–85 °C with 20-second exposure to the cornea. In the experimental group (right eyes of rabbits) the animals were treated with developed 6-methyl-3-(thietane-3-yl)uracil eye ointment, in the control group (left eyes of rabbits) 20% gel of solcoseryl was applied. Animals were observed daily for 14 days. The condition and the morphological changes of the cornea were compared on days 1, 3, 7, 10, and 14 after the experimental thermal burn. Results. The features of reparative processes after corneal thermal burn depending on used treatment were determined. The study revealed that 6-methyl-3-(thietane-3-yl)uracil improved trophics more repidly, increased cell growth and cell reproduction, stimulated the regeneration process in damaged corneal tissues, accelerated wound and burn healing, and also provided anti-inflammatory effect. Positive dynamics was registered on days 4–5 in the experimental group, and by the 7th day in the control group. With 6-methyl-3-(thietane-3-yl)uracil no toxic reactions in the form of destruction of the collagen skeleton, maceration, pronounced edema and hyperemia were observed. On day 14 of follow-up, the microscopic picture of rabbits’ cornea had the usual normal structure. Conclusion. Treatment with 6-methyl-3-(thietane-3-yl)uracil resulted in faster and more structured and anatomically correct healing of corneal thermal burn compared to the treatment with alternative medication solcoseryl in the control group.