Effect of subchronic intoxication with thyram on the activity of antioxidant enzymes and the state of lipoperoxidation processes

Abstract

AIM: This study aimed to investigate the effect of oxidative stress on the antioxidant defense system and state of lipid peroxidation of the body. MATERIAL AND METHODS: Modeling of oxidative stress was carried out on 240 rats by daily administration of thiram at a dose of 1/50 LD50 (8 mg/kg body weight) in the diet of rats for 4 weeks. The catalytic activity of superoxide dismutase and catalase and the concentration of malondialdehyde and diene conjugates were analyzed. RESULTS: The modeling of subchronic intoxication significantly decreased the activity of catalase and superoxide dismutase (p 0.05) and increased the concentration of malondialdehyde, diene conjugates in blood plasma, erythrocyte mass, and liver homogenate. After experimental subchronic intoxication, antioxidants such as vitamin E at a dose of 8.58 mg/kg and milk thistle extract at a dose of 13.74 mg/kg were used. Using such antioxidants within 30 days had significantly restored the activity of superoxide dismutase and catalase, and it also reduced the concentration of malondialdehyde and diene conjugates in all investigated body media. CONCLUSION: Fungicide intoxication with thiram affects redox homeostasis. In addition, the usage of vitamin E and milk thistle extract can restore the prooxidantantioxidant balance of the body.